Cancer Lett 2011 Dec;313 (2): 192-200. [IF:4.864]
A possible role of cIAP2 in Helicobacter pylori-associated gastric cancer.
Li Z , Chen J , Chan KW , Qiao L , Wong BC .
Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, 3002 Shungang West Road, Futian District, Shenzhen 518035, Guangdong Province, China.
深圳市第二人民医院，深圳大学第一附属医院
Abstract
Cellular inhibitor of apoptosis protein 2 (cIAP2) is a member of the IAP family and is over-expressed in most cancer tissues. In this study, we investigated the role cIAP2 in Helicobacter pylori (HP) related gastric carcinogenesis. We measured the expression of cIAP2 at mRNA and protein levels in a panel of gastric cancer cell lines and human gastric cancer tissues by semi-quantitative reverse transcriptase PCR (RT-PCR), quantitative real time PCR (qPCR), immunoblotting, and immunohistochemistry. The effects of cIAP2 down-regulation on gastric cell proliferation and apoptosis were detected by standard WST-1 assay and flow cytometry, respectively. Infection of gastric mucosa by HP enhances the expression of cIAP2 in mouse gastric tissues. Over 70% of human gastric cancer tissues express higher amount of cIAP2. Well-differentiated gastric cancer cells express more cIAP2 than moderately- and poorly-differentiated gastric cancer cells. Knocking down of cIAP2 in SGC-7901 cells results in a 30% decrease in cell proliferation, a 20% increase in apoptosis and delayed migration. Thus, cIAP2 may play an important role in HP-induced gastric carcinogenesis, and it may serve as a potential target for gastric cancer therapy.
摘要:
细胞抑制因子凋亡蛋白2(cIAP2)是IAP家族成员之一，在多数肿瘤组织中过表达。在本实验中，我们探讨cIAP2在幽门螺旋杆菌(HP)相关胃癌中的作用。我们通过半定量反转录PCR(RT-PCR)、定量实时PCR(qPCR)、免疫印迹和免疫组化等方法在mRNA和蛋白水平检测胃癌细胞系和人胃癌组织中cIAP2的表达情况。分别用标准WST-1细胞增殖检测试剂盒和流式细胞仪检测下调cIAP2对胃癌细胞增殖和凋亡的影响。感染胃粘膜的HP促进cIAP2在大鼠胃粘膜中的表达。超过70%的人胃癌组织中高表达cIAP2。高分化的胃癌细胞中cIAP2是表达高于中分化和低分化胃癌细胞。在SGC-7901中敲除cIAP2导致细胞增殖降低30%、凋亡和细胞延迟迁移增加20%。因此，cIAP2在HP相关胃癌中可能起重要作用，有可能是胃癌治疗的一个潜在的靶点.
